Fig. 6

Expression levels of porphyrin and chlorophyll metabolism-related genes by RT-qPCR and RNA-seq. The heat map shows the FPKM values for the 23 selected candidate genes. Sample names are displayed below the column charts. Column height indicates the fold change in gene expression from the RT-qPCR results. RT-qPCR was performed with the primer sets in Table 2, and GAPDH was used as the reference gene. The error bars on each column indicate the SD based on three replicates. Different lowercase letters above the bars indicate statistically significant differences at P < 0.05 (one-way ANOVA, Duncan’s tests). Different letters indicate significant differences between groups, while the same letter represents no significant difference. The genes and the gene_ids are as follows: HEPH (ncbi_101803569); UROS (ncbi_101803009); UROD (ncbi_101794950); UGT1–1-like (ncbi_101802378); protohaem IX farnesyltransferase 2 (ncbi_101790381); MMAB (ncbi_101804472); HMOX2 (ncbi_101801310); HMBS (ncbi_101799203); FXN (ncbi_101800676); EARS2 (ncbi_101794546); CP (ncbi_101795788); CPOX (ncbi_101804003); COX15 (ncbi_101800616); ALAD (ncbi_101797189); FECH (ncbi_101796583); ALAS1 (ncbi_101793120); EPRS glutamyl-prolyl-tRNA synthetase (ncbi_101794263); HMOX1 (ncbi_101805057); BLVRA (ncbi_101793149); GUSB glucuronidase beta (ncbi_101793510); Cytochrome c-type haem lyase (ncbi_101802937); UGT2A2 (ncbi_101796668); Protohaem IX farnesyltransferase (ncbi_101790381)