Fig. 5

Hybrid CRISPR-Cas type III-B/I-F system associated with a prophage in V. cholerae and V. metoecus. a Genome comparison of V. cholerae N16961 and HE-45. The hybrid system is at the 3′ region of a prophage located between VCA0884 and VCA0885 relative to the reference strain N16961, which lacks the region. A homologous phage was found in V. metoecus RC341 and was inserted at the same genomic location. b The conserved protospacer adjacent motif (PAM) was identified by analyzing the protospacers and is represented using Weblogo. c The hybrid system consists of type III-B cas7cas10cas5cas7cmr5cas7 followed by a resolvase and cas6f and a type I-F CRISPR. The core genes are colored in black, putative phage genes are colored in light blue, putative integrases are colored in red. Genomic comparisons and gene arrangements were constructed in Easyfig. Grayscale bars represent nucleotide homology. Inverted triangles represent a 156-bp direct repeat