Fig. 6

DNA gel electrophoresis of the qPCR products showing that the primers were specific in amplification. Panel a: L) DNA ladder; 1) GAL3ST2 (177 bp); 2) CYP7A1 (171 bp); 3) CALB1 (116 bp); 4) TYRO3 (186 bp); 5) CNG4 (243 bp); 6) POMC (115 bp); 7) RHOBTB3 (171 bp); 8) KCNH1 (160 bp); 9) MMP13 (128 bp); 10) KLB (224 bp); 11) GATA3 (157 bp); 12) SPP1 (134 bp). Panel b: L) DNA ladder; 1) YWHAZ (61 bp); 2) TBP (147 bp); 3) IBV T-as positive control (181 bp); 4) CA9 (80 bp); 5) OTOP2 (202 bp); 6) CGA (166 bp); 7) CLDN16 (186 bp); 8) GJA8 (186 bp); 9) SS2 (160 bp); 10) BPIFB3 (234 bp); 11) PPARGCIB-Positive control (82 bp); 12) TLR3-Positive control (203 bp). The upper (purple) and lower markers (green) act as internal standards and are used to align the ladder analysis with the individual DNA sample analysis. The DNA gel in Agilent 2100 Bioanalyzer was performed as per the manufacturer’s instructions of Agilent DNA 1000 Kit. The size of the individual amplicons are consistent with the expected size