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Table 4 Top 50 up-regulated DEGs at 15 h relative to 5 h time-point

From: RNA-sequencing analysis of shell gland shows differences in gene expression profile at two time-points of eggshell formation in laying chickens

Gene symbol

Gene name

Fold change

FDR

POMC

Proopiomelanocortin

+ 9.179

0.0005

CALB1

Calbindin

+ 8.081

3E-08

SPP1

Secreted phosphoprotein 1

+ 7.993

2E-07

NEU4

Neuraminidase 4

+ 7.682

0.0021

CEMIP

Cell migration inducing hyaluronan binding protein

+ 7.555

9E-06

GAL3ST2

Galactose-3-O-sulfotransferase 2

+ 6.999

0.0005

SLC6A17

Solute carrier family 6 member 17

+ 6.643

0.0271

GNG4

G protein subunit gamma 4

+ 6.586

0.0416

BPIFB3

BPI fold containing family B, member 3

+ 6.414

3E-06

ECEL1

Endothelin converting enzyme Like 1

+ 6.408

0.0312

REG4

Regenerating family member 4

+ 6.272

0.0017

ANGPTL3

Angiopoietin like 3

+ 6.199

0.0002

LOC415478

Transmembrane protein 2-like

+ 6.062

6E-06

KCNH1

Potassium voltage-gated channel, subfamily H (eag-related), member 1

+ 5.956

0.0009

GNRHR

gonadotropin-releasing hormone receptor

+ 5.949

0.0122

MKI67

Marker of proliferation Ki-67

+ 5.815

0.0127

BPIL3

Bactericidal/permeability-increasing protein-like 3

+ 5.250

0.0004

SLC29A4

Solute carrier family 29 member 4

+ 5.183

8E-05

WNT11

Wnt family member 11

+ 4.799

4E-05

CHRD

Chordin

+ 4.691

3E-05

OFCC1

Orofacial cleft 1 candidate gene 1 protein homolog

+ 4.330

0.0005

GPR183

G Protein-coupled receptor 183

+ 4.329

0.0001

ETV4

ETS variant 4

+ 4.309

0.0264

RHOBTB3

Rho related BTB domain containing 3

+ 4.305

0.0036

OTOP2

Otopetrin 2

+ 4.226

1E-05

MFSD13A

Major facilitator superfamily domain containing 13A

+ 4.190

2E-08

TNFRSF6B

TNF receptor superfamily member 6b

+ 4.182

0.0005

PLPPR4

Phospholipid phosphatase related 4

+ 4.161

8E-05

B3GNT7

BetaGal beta-1,3-N-acetylglucosaminyltransferase 7

+ 4.139

9E-05

SEMA3D

Semaphorin 3D

+ 4.126

0.0206

FAM163A

Family with sequence similarity 163 member A

+ 4.125

0.004

RUBCNL

RUN and cysteine rich domain containing beclin 1 interacting protein like

+ 4.049

1E-06

OTOP3

Otopetrin 3

+ 4.003

0.0014

BHLHA15

Basic helix-loop-helix family member a15

+ 3.998

0.0354

SLC38A8

Solute carrier family 38 member 8

+ 3.941

0.0013

TUBB3

Tubulin beta 3 class III

+ 3.924

0.0002

ETNK1

Ethanolamine kinase 1

+ 3.918

4E-06

MIR6556

Gga-mir-6556

+ 3.913

0.0017

PERM1

PPARGC1 and ESRR induced regulator, muscle 1

+ 3.902

0.007

COL21A1

Collagen type XXI alpha 1 chain

+ 3.885

0.0003

NPTX1

Neuronal pentraxin 1

+ 3.884

0.0106

EREG

Epiregulin

+ 3.867

0.0434

FABP3

Fatty acid binding protein 3

+ 3.824

4E-05

SS2

Somatostatin II

+ 3.804

0.0062

PHGDH

Phosphoglycerate dehydrogenase

+ 3.777

0.0004

HEPACAM

Hepatic and glial cell adhesion molecule

+ 3.751

0.0055

MAP 3 K15

Mitogen-activated protein kinase kinase kinase 15

+ 3.737

1E-05

WSCD2

WSC domain containing 2

+ 3.716

6E-07

NKAIN1

Na+/K+ transporting ATPase interacting 1

+ 3.694

0.0038

PTN

Pleiotrophin

+ 3.693

0.0016

  1. Fold change (FC) was calculated in log2 value. Plus (+) sign shows up-regulation of the genes at 15 h relative to 5 h time-point