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Table 5 Putative functions of mRNA sequences that did not annotate to any known gene IDs during Ensembl annotations of RNA-Seq transcripts

From: RNA-sequencing analysis of shell gland shows differences in gene expression profile at two time-points of eggshell formation in laying chickens

Group

Sequence ID

Gene ID

Associated GO term

Fold change

FDR

aG1

ENSGALG00000039411

COL25A1

Heparin binding and beta-amyloid binding

− 4.899

0.0031

ENSGALG00000032113

NAT8L

N-acetyltransferase activity and aspartate N-acetyltransferase activity

− 4.038

9E-05

ENSGALG00000029321

NAT8L

N-acetyltransferase activity and aspartate N-acetyltransferase activity

− 3.857

0.0009

ENSGALG00000030322

TMEM163

No associated GO term found

− 3.349

0.0003

ENSGALG00000038759

PARD6B

No associated GO term found

− 2.867

0.0007

bG2

ENSGALG00000037163

SLC6A4

Protein homodimerization activity and Rab GTPase binding

+ 6.402

0.0004

ENSGALG00000006393

ADGRG6

G-protein coupled receptor activity and transmembrane signaling receptor activity

+ 5.243

0.0306

ENSGALG00000039812

GPR6

G-protein coupled receptor activity and sphingosine-1-phosphate receptor activity

+ 5.154

0.0014

ENSGALG00000029410

NR1D1

Transcription factor activity, sequence-specific DNA binding and RNA polymerase II core promoter proximal region sequence-specific DNA binding

+ 4.578

0.0031

ENSGALG00000041414

BHLHE41

Protein homodimerization activity and RNA polymerase II core promoter proximal region sequence-specific DNA binding

+ 4.419

0.0003

ENSGALG00000042845

PDE3A

3,5-cyclic-nucleotide phosphodiesterase activity and 3,5-cyclic-AMP phosphodiesterase activity

+ 3.885

0.0039

ENSGALG00000035935

UNC13C

Diacylglycerol binding

+ 3.701

7E-06

ENSGALG00000033066

UBE2E2

Ligase activity and acid-amino acid ligase activity

+ 3.672

0.0166

ENSGALG00000033883

PCDH7

Calcium ion binding

+ 3.125

0.0042

ENSGALG00000031565

ZNF277

RNA polymerase II core promoter sequence-specific DNA binding

+ 3.005

0.0399

ENSGALG00000037545

GRIP2

Protein C-terminus binding and receptor signaling complex scaffold activity

+ 2.937

0.0009

ENSGALG00000008047

TP53I11

Ligase activity and ubiquitin protein ligase activity

+ 2.903

0.0216

ENSGALG00000011860

MYO16

Actin binding and actin filament binding

+ 2.596

0.0036

ENSGALG00000042801

NT5DC4

Hydrolase activity and 5-nucleotidase activity

+ 2.396

0.0298

ENSGALG00000039716

HPCA

Calcium ion binding and actin binding

+ 2.304

1E-05

ENSGALG00000041604

NPTXR

No associated GO term found

+ 2.295

0.0051

ENSGALG00000030673

KCTD14

NADH dehydrogenase (ubiquinone) activity

+ 2.096

0.0076

ENSGALG00000042104

ROBO1

Identical protein binding and LRR domain binding

+ 2.044

0.0119

ENSGALG00000038532

ESPN

Actin binding and SH3 domain binding

+ 1.878

0.0086

ENSGALG00000038993

NEGR1

No associated GO term found

+ 1.842

3E-06

ENSGALG00000041238

SEMA3B

Receptor activity

+ 1.798

3E-05

ENSGALG00000042411

FAM198B

No associated GO term found

+ 1.765

0.0009

ENSGALG00000043703

ELN

Extracellular matrix structural constituent and extracellular matrix constituent conferring elasticity

+ 1.630

0.0034

ENSGALG00000043198

DNPEP

Metallopeptidase activity and aminopeptidase activity

+ 1.609

0.0021

ENSGALG00000043209

ADGRB2

G-protein coupled receptor activity and transmembrane signaling receptor activity

+ 1.573

0.0267

  1. To retrieve the best homology hit, the sequence IDs were blasted against chicken, duck, turkey and human reference genomes in Ensembl BLAT database. The cut off criterion was established as e-value <10E− 20. aRepresents genes significantly (log2 fold change > 1.5; FDR < 0.05) down-regulated at the 15 h relative to the 5 h time-point. bRepresents genes significantly up-regulated at the 15 h relative to the 5 h time-point