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Table 6 Comparison of the gene expression data between RNA-Seq and qPCR

From: RNA-sequencing analysis of shell gland shows differences in gene expression profile at two time-points of eggshell formation in laying chickens

Gene

Fold change

qPCR

RNA-Seq

CGA

−2.335

−3.078

CYP7A1

−10.374

− 4.170

GATA3

−7.331

−3.036

GJA8

−19.366

−4.522

MMP13

−4.320

−4.673

TYRO3

−4.249

−1.942

CA9

−10.377

−4.519

KLB

−38.019

−5.998

GNG4

+ 5.427

+ 6.586

KCNH1

+ 32.199

+ 5.956

BPIFB3

+ 30.038

+ 6.414

POMC

+ 316.268

+ 9.179

SPP1

+ 306.140

+ 7.993

GAL3ST2

+ 68.867

+ 6.999

OTOP2

+ 12.366

+ 4.226

CALB1

+ 74.817

+ 8.081

SS2

+ 13.025

+ 5.434

CLDN16

+ 5.378

+ 3.212

RHOBTB3

+ 14.644

+ 4.305

  1. For qPCR, the relative expression level of genes at the 5 h and 15 h time-points was calculated in qbase+ software based on 2^-ΔΔCq with genes amplification specific efficiency. For gene expression data normalisation, TBP and YWHAZ were used as reference genes. Plus and minus signs show down- or up-regulation of genes at the 15 h relative to the 5 h time-point
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BMC Genomics

ISSN: 1471-2164

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