Fig. 6
Validation of the DEGs expression exposed to carbendazim using qRT-PCR. Ten genes associated with transmembrane transport (a), response to stress (b), the target gene of carbendazim (c) and oxidoreductase activity (d), were selected for qRT-PCR analysis. (e) Pearson correlation of fold change analyzed between qRT-PCR and RNA-Seq. SJ51_C and SJ51M_C represented without carbendazim treatment, while SJ51_E and SJ51M_E represented exposed to carbendazim treatment. The mRNA abundance was normalized using the housekeeping actin gene, and the gene relative expression levels are represented by the log2Ratio. Data of qRT-PCR are presented as mean ± SD (n = 9)