Fig. 2
Differential expression of PDIM cluster genes in M. tb Comp1. a Hierarchical clustering of whole transcriptomes of M. tb H37Rv, Mut1 and Comp1. (Expression values are shown as mean fold change from 3 replicates normalized with respect to M. tb genomic DNA; GEO dataset Accession number GSE30264). b Heat map depicting expression values of genes belonging to the PDIM cluster, extracted from the microarray data shown in panel A. The vertical arrows on the left depict the arrangement of operons in the PDIM biosynthesis cluster [8]. The horizontal arrows on the right depict genes that were expressed at a lower level as compared to H37Rv. c RT-qPCR analysis of selected PDIM genes in M. tb strains. Relative transcript levels were calculated from Ct values normalized for 16S rRNA expression and then expressed with respect to that of H37Rv. Mean transcript level ± SD determined from three independent cultures is shown. [**, pvalue < 0.01 and ***, pvalue < 0.001 in comparison to H37Rv]