Genome analysis identifies a spontaneous nonsense mutation in ppsD leading to attenuation of virulence in laboratory-manipulated Mycobacterium tuberculosis

Table 2 SNPs unique to M. tuberculosis Comp1 strain^a

SNP location	Rv number	Gene Name	Base change	Amino Acid Change	Gene Description	Protein Function	Type (N/S)^b
161047	Rv0133		G/A	G60D	Acetyltransferase	Acetylation, substrate unknown	N
608331	Rv0516c		A/C	M69 L	Conserved hypothetical protein	May be involved in regulating sigma factor	N
635633	Rv0543c		C/T	A81A	Conserved hypothetical protein	Function unknown	S
958922	Rv0861c	ercc3	C/A	A410A	DNA helicase 3	Involved in nucleotide excision repair	S
1711627	Rv1520		C/T	Y200Y	Conserved hypothetical protein	Function unknown	S
2006032	Rv1771		A/G	Q291R	Oxidoreductase	Possibly involved in biosynthesis of L-ascorbic acid (vitamin C). Oxidizes L-gulono-1,4-lactone.	N
3267020	Rv2934	ppsD	G/A	W1591Stop	Phenolpthiocerol synthesis type-I polyketide synthase	Involved in phenolphthiocerol and phthiocerol dimycocerosate (dim) biosynthesis	N
3948356	Rv3514	PE_PGRS57	A/G	S855G	PE-PGRS family protein PE_PGRS57	Function unknown	N

^aThe SNPs unique to Comp1 were identified by comparing the whole genome sequences of Comp1 vs. H37Rv strain
^bN Non-synonymous mutation, S Synonymous mutation

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