Fig. 3
From: De novo assembly of Persea americana cv. ‘Hass’ transcriptome during fruit development
Differentially Expressed Genes analsyis. Profiling expression of DEG in P. americana cv. ‘Hass’(a) Heatmap of DEGs (FC ≥ 1 and FDR ≤ 0.01) across all four developmental stages, resulting in 8672 differentially expressed genes (DEGs). Y-axis corresponds to the different genes used as input information, X-axis shows the different conditions with their respective biological replicates. The color key represents the median centered log2 TMM-normalized TPM values. b Subcluster Plots, three out of fifteen (K = 15) subclusters were used to find candidate genes because of their expression profiles. The X-axis shows the four developmental stages and the Y-axis is the median centered log2 expression. c Validation of gene expression by qPCR for selected genes from subclusters 4, 7 and 11: CBV_PAM10189 (subcluster 4), CBV_PAM17119 (subcluster 7) and CBV_PAM1355 (subcluster 11). The Y-axis displays relative expression levels to PamActin gene, while the X-axis indicates the development stage