Fig. 2

Histopathological assessment of genital tract of mice infected twice with Chlamydia. a. Representative image of the endometrium from an uninfected mouse. A single layer of columnar epithelia (arrow), with basally located nuclei lining the endometrium. The underlying lamina propria has scattered spindled cells, some capillaries, and occasional leukocytes in a pale basophilic myxomatous matrix. (Endometrial gland = Gl). HE stain. Scale bar 50 μm. b. Representative image of uterine endometrium with periglandular fibrosis after 1 week of reinfection. Increased numbers of neutrophils (asterisk) and spindled cells (fibroblasts, arrow) are arranged around endometrial glands (Gl). HE stain. Scale bar 50 μm. c. Representative image of uterine endometrium with increased lymphocyte presence after 2 weeks of reinfection. Large numbers of lymphocytes (arrows) obscure and expand the tissues around an endometrial gland (Gl). HE stain. Scale bar 50 μm. d and e. Representative image of uterine endometrium with increased lymphocyte presence after 4 weeks of reinfection with lymphocytic endometritis. Large numbers of lymphocytes, with fewer plasma cells (long arrows) and occasional neutrophils (short arrow) obscure and expand the tissues around an endometrial gland (Gl). Arrowheads point to foci of glandular apoptotic necrosis. Capillaries = Ca. HE stain. Scale bars 50 and 20 μm, respectively. f. Representative image of uterine endometrium with increased lymphocyte presence and lymphoid follicle formation (arrows) after 8 weeks of reinfection. An expansive lymphoid follicle obscures and expands the tissues of the deep stratum spongiosum and adjacent myometrium. HE stain. Scale bar 50 μm