Fig. 4

Differential gene expression between CCI and naïve groups in female rats. a Schematic diagram of experiment. Female rats were randomly assigned to the naïve group or receive CCI. RNA-seq performed on ipsilateral L4-L6 DRGs from each animal. Differentially expressed genes (DEG) are defined as genes expressed after CCI versus naïve with a |log₂FC| > 0.5 and an FDR < 0.05. b Volcano plot showing RNA-seq data of DRGs from naïve female rats and male rats following CCI. DEGs are designated in red. Triangles represent genes with extremely high log₁₀FDR and/or log₂FC values. c Gene ontology pathways associated with increased (top) and decreased (bottom) differential expression in CCI versus naïve. d Heatmap that shows the log₂FC, log₁₀(gene count + psuedocount of 1), and the –log₁₀FDR for the upregulated genes enriched in the top 2 GO terms shown in c). DRG dorsal root ganglia, CCI chronic constriction injury, FC fold change, FDR false discovery rate