Fig. 2

Amino acid sequences of the complete VWamide preprohormone from T. cystophora, A. alata, C. xaymacana, and C. fleckeri. Residues and peptide sequences are highlighted as in Fig. 1. The VWamide preprohormone from T. cystophora (named Tcy-VWamide) contains six copies of Tcy-VWamide-1 (pQPPGVWamide), which are preceded by wither S, T, or A residues. The VWamide preprohormone from A. alata contains six copies of a neuropeptide identical to Tcy-VWamide-1, which are preceded by either S, T, or R residues. The VWamide preprohormone from C. xaymacana contains five copies of Tcy-VWamide-1. Each copy is preceded by either S, or T residues. The VWamide preprohormone from C. fleckeri contains four copies of Tcy-VWamide-1, one copy of a peptide with the PAamide C-terminal sequence (pQSPAamide), and one copy of a peptide with the NWamide C-terminal sequence (pQGNWamide)