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Fig. 5 | BMC Genomics

Fig. 5

From: The NAC family transcription factor GmNAC42–1 regulates biosynthesis of the anticancer and neuroprotective glyceollins in soybean

Fig. 5

GmNAC42s expressions are induced with glyceollins in response to abiotic and biotic elicitors. a, b Gene expressions following treatment with pH 3.0 medium at 9 dat or dehydration at 6 dat relative to their respective controls measured by qRT-PCR. aSignificantly greater and bsignificantly less than control, paired students t-test (P < 0.01). c Unrooted phylogenetic tree of GmNAC42 amino acid sequences and characterized NACs. GenBank Accessions and Phytozome landmarks: GmNAC42–1 (KRH73619; Glyma.02G284300), GmNAC42–2 (KRH14512; Glyma.14G030700), GmNAC42–3 (KRG98971; Glyma.18G110700), VvNAC042_5 (XP_002283251; VIT_12s0028g00860), ANAC042 (Q9SK55; AT2G43000), SlJUB1 (XP_019069297; Solyc05g021090); AtLOV1/ANAC035 (Q9ZVP8.2; AT2G02450), DlNAC1 (ABQ96120; N/A), ANAC032 (AAM65083; AT1G77450), GmNAC32–1 (NP_001236871.2; Glyma.06G114000), GmNAC32–2 (NP_001240958.1; Glyma.04G249000), StNTP1 (AGY49284; PGSC0003DMT400001498), StNTP2 (AGY49285; PGSC0003DMT400079997), NbNTP2 (AGY49287; N/A), SlSRN1 (NP_001304297; Solyc12g056790), GmNTP1–1 (NP_001276287; Glyma.02G222300), GmNTP1–2 (XP_006596412; Glyma.14G189300), GmNTP2–1 (XP_003556180; Glyma.20G172100), GmNTP2–2 (NP_001274375; Glyma.10G219600). The number adjacent branches indicate maximum parsimony bootstrap values for the corresponding node. The scale bar indicates the number of differences per 100 residues derived from the Muscle alignment. The phylogenetic tree was generated using MEGA v5.0 software [77]. d Amount of total glyceollins extracted from empty-vector transformed soybean hairy roots following treatment with H2O or wall glucan elicitor (WGE) from P. sojae. e Gene expressions following 24 h of WGE or water treatment of soybean hairy roots measured by qRT-PCR

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