Fig. 7

Nuclear localization and DNA binding activities of GmNAC42–1. (a-f) Confocal fluorescence microscopy images of GFP-GmNAC42–1 fusion protein in transgenic soybean hairy roots. a-c Root cell expressing GFP-GmNAC42–1 fusion protein. d-f Root cell expressing GFP. a Propidium iodide (10 μg mL^− 1) staining the plasma membrane and nucleus. b GFP-GmNAC42–1 fluorescence at plasma membrane and nucleus. c Overlay of GmNAC42–1 and propidium iodide fluorescence from panels a and b. d Propidium iodide staining. e GFP signal in cytosol and other extranuclear compartments. f Overlay of GFP and propidium iodide fluorescence from D and E. Bars = 10 μm. g Schematic diagram demonstrating G4DT and IFS2 promoter fragments used for yeast one-hybrid assays and predicted NAC binding elements (blue boxes). h Yeast one-hybrid assays of YM4271 yeast transformed with GmNAC42–1 on SD/−His/−Leu medium containing various concentrations of 3-AT. Arrows: gray indicates weak binding, white no binding, and black strong binding