Fig. 1

Analysis of the structure and expression of the LINC01420 transcript. a. RT-PCR analysis of nine LINC01420 loci of total RNA isolated from HeLa cells (H) and human skin fibroblasts (F) above. The bold numbers (1–9) indicate the amplified locus. The numbers above horizontal arrows show the length of observed products in base pairs (bp). Scheme of the ResSeq (NM_001348129.1) and two experimentally established (MH892397, MH892398) isoforms of the LINC01420 transcript with the amplified loci are shown below. Results of 5′- and 3′- RACE analysis is presented under the scheme. The vertical arrows represent the genomic position of exact 5′- and 3′-ends. Nucleotide numbering was based on reference sequence NM_001348129.1 b. RT-qPCR analysis revealed expression level of three LINC01420 loci relative to four reference genes (HPRT1, B2M, TFRC, TBP) in 12 human cell lines and human skin fibroblasts. Loci 1 and 3 are common for short and long isoforms, whereas locus 5 is long isoform specific. For illustrative purposes expression data are given in logarithmic coordinates. c. To identified the subcellular localization of the LINC01420 transcript total RNA was extracted from three separated cell fractions: cytoplasmic (cyto), nuclear-soluble (nuc.sol) and chromatin-bound (chroma) of HEK293T cells. The amount of transcript in the different fractions relative to whole-cell RNA was measured by RT-qPCR. The error bars represent SEM (standard error mean)