Table 1 Primer sequences used for amplification of different LINC01420 loci and reference genes and siRNA sequences used for knockdown
From: LINC01420 RNA structure and influence on cell physiology
Primer | Sequence |
|---|---|
F1 | 5′ - GCCCACCGGAGAAAACTGAC – 3 |
R1 | 5′ – CCTTCCGGATAATCCCAACG - 3‘ |
R1–1 | 5′ – GAGGCTTGGCTTCCCGTG - 3‘ |
R1–2 | 5′ – TTCCAGGTGGGAGAGTGGA – 3 |
F2 | 5′ – AGTGATTGCAGTATGACTCCA - 3‘ |
R2 | 5′ – TTCCAGGTTCAGGACACCAGA - 3‘ |
F3 | 5′ – TCAGCGCGATTTCACTTCCTG – 3’ |
F3–1 | 5′ – CTGAATTTCGATGAATTCTAAGAC – 3’ |
F3–2 | 5′ – ACCTCTGAGATTTAAGGCCATG – 3’ |
R3 | 5′ – GACCATCTCACAGGCATTGTT – 3’ |
F4 | 5′ – GTACACTTTCTTTAATTTGCTGTC – 3’ |
F5 | 5′ – GAAAATGTCAGATAAACTTGGCT – 3’ |
R5 | 5′ – CTTTAATTCTGATGCTAGGGACT – 3’ |
HPRTf | 5′ – TGTAATGACCAGTCAACAGGG - 3’ |
HPRTr | 5′ – TGCGACCTTGACCATCTTTG - 3’ |
B2Mf | 5′ – TCTTTCAGCAAGGACTGGTC - 3’ |
B2Mr | 5′ – GGCATCTTCAAACCTCCATG - 3’ |
TBPf | 5′ – CGGAGAGTTCTGGGATTGTAC - 3’ |
TBPr | 5′ – GTGGTTCGTGGCTCTCTTATC - 3’ |
TFRCf | 5′ – TCCTTGCATATTCTGGAATCCC - 3’ |
TFRCr | 5′ – ATCACGAACTGACCAGCG - 3’ |
siLINC01420 | 5′-UCCGGAGAAGUAGAGAAAUdTdT-3′/ 5′-AUUUCUCUACUUCUCCGGAdTdT-3′ |
siEIF3D#1 | 5′-GCGUCAUUGACAUCUGCAUdTdT-3′/ 5′-AUGCAGAUGUCAAUGACGCdTdT-3′ |
siEIF3D#2 | 5′-CGACAUGGAUAAGAAUGAAdTdT-3′/ 5′-TTCATTCTTATCCATGTCGdTdT-3’ |
siControl | 5′-AGGUAGUGUAAUCGCCUUGdTdT-3′/ 5′-CAAGGCGAUUACACUACCUdTdT-3′ |
FAM-control | 5′-FAM-AGGUCGAACUACGGGUCAAdTdT-3′/ 5′-FAM- UUGACCCGUAGUUCGACCUdTdT-3′ |