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Fig. 6 | BMC Genomics

Fig. 6

From: Delineating the role of c-FLIP/NEMO interaction in the CD95 network via rational design of molecular probes

Fig. 6

Experimental validation of the peptides. a Peptides were covalently bound to beads and incubated with lysates from HeLa-CD95-FRL cells. Binding of c-FLIP was analyzed with immunoblotting for c-FLIP. b Apoptotic/non-apoptotic signaling in HeLa-CD95-FL cells upon CD95L stimulation for one and three hours (h) was analyzed via immunoblotting. Immunoblotting has been performed using the indicated antibodies. As the hallmarks of apoptosis induction the processing of procaspase-8a/b, procaspase-10d/a and procaspase-3 to their cleavage products has been analyzed. As the hallmark of NF-κB activation, the phosphorylation and degradation of IκBα were monitored. Actin was used as a loading control. One representative experiment out of three independent experiments is shown. c Analysis of the peptide effects on CD95-mediated NF-κB activation via IL8 ELISA analysis

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