Fig. 3

Detection of close relative viruses irrespective of extensive multiplexing. Sequencing libraries made from serum samples spiked with ZIKV, DENV, CHIKV and/or HAdV were prepared in duplicate and probed singly or probed in pools of four or 12. a, c, e, g The percentage of reads that map to each strain of spiked-in ZIKV, DENV, CHIKV, and HAdV, respectively. Each co-infected sample is denoted with an asterisk (*). Estimated genome equivalents per mL as extrapolated from RT-qPCR standard curves are listed along the top of each graph. The standard error of the mean of two replicates is shown. b, d, f, h Coverage plot for replicate one of each ZIKV-, DENV-2-, CHIKV-, and HAdV-containing sample, respectively