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Fig. 4 | BMC Genomics

Fig. 4

From: Enrichment post-library preparation enhances the sensitivity of high-throughput sequencing-based detection and characterization of viruses from complex samples

Fig. 4

Recovery of full DENV genome at titers below limit of detection by conventional shotgun HTS or amplicon-based sequencing. DENV-1 and DENV-2 RNA were spiked into human serum RNA at a range of GE corresponding to Ct 26–32, in duplicate, and libraries were prepared using target enrichment in pools of four. Corresponding estimated genome equivalents per mL as extrapolated from a standard RT-qPCR curve are listed below the axis. Mock samples consisted of human serum RNA extract only. a The proportion of total reads that map to DENV-1 or DENV-2 at each Ct value. Error bars show standard error of two replicates. b-c The proportion of reads that map to DENV-1 or DENV-2, respectively, at each spike-in level. Bubble size corresponds to depth of coverage of the viral genome (average of two replicates)

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