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Fig. 7 | BMC Genomics

Fig. 7

From: Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes

Fig. 7

Cardiomyocytes (D15) showed down-regulation of translation and RNA processing genes. (a) GO EnrichR BP RNA-related terms enriched for D15 down-regulated genes (FDR < 0.05, logFC<− 1) compared to hESC (D0). (b) Genes classified on RNA-related BP terms (a) according to the co-regulated, buffered and loaded classification as indicated. (c) KEGG pathway analysis of D15 down-regulated ribosomal proteins. Down-loaded proteins showed in solid color, down co-regulated proteins showed as a green outline. (d) Venn diagram and (e) GO EnrichR BP enriched terms of polysome recruitment (FDR < 0.05, logFC> 2) or dissociation (FDR < 0.05, logFC<− 2) for non-DEG based on polysome/ribosome-free ratio of D0 vs. D15. (f) Translation genes showed polysome/ribosome-free ratio decreasing on D15 (FDR < 0.05, logFC<− 2). (g) Representative images of D0 and D15 cells cultured with OPP to stain nascent proteins. (h) Quantification of Alexa488 fluorescence intensity (OPP incorporation) at the cytoplasm region around the nucleus. For each condition (D0 and D15), 1400 cells were randomly chosen for intensity analysis. Statistical analysis was performed using the Mann-Whitney test (nonparametric t test). ****p < 0.0001

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