Fig. 7
From: Systems based analysis of human embryos and gene networks involved in cell lineage allocation
Summary schematic representing 8-cell blastomere genomic divergence. Using a combination of systems biology techniques including network analysis, nearest neighbour PCA (isoMap), EIF expression and epigenetic-associated gene expression we have detected two sets of blastomeres within an 8-cell embryo which exhibit diverging tendencies towards ICM and TE. Nearest neighbour PCA analysis (isoMap) reveals similarities in global gene expression between blastomere 3, 4, 7, 1, 6 and 5 with Inner Cell Mass (Blue brackets/arrows), and blastomere 8 and 2 with Trophectoderm (burgundy brackets/arrows). Green arrows represent similarity in network modules, as highlighted by circos plots. Black bracketed Blastomere 3, 7 and 8 are enriched in RNAseq cluster 3 and Blastomere 6, 5 and 8 are enriched in RNAseq cluster 1. Red brackets/arrows represent blastomeres with the greatest maintenance and loss of developmental EIF expression. B3, B4 and B6 (red coloured blastomeres) have distinct epigenetic associated gene expression signatures from those expressed by blastomeres B1, B2, B5, B7 and B8 (blue blastomeres)