Fig. 8

PPARα deficiency mitigates effect of CREB3L3 deficiency on hepatic gene expression during ketogenic diet. a Volcano plot showing the relation between signal log ratio (²log[fold-change], x-axis) and the -¹⁰log of the IBMT P-value (y-axis) for the comparison between wild-type mice and PPARα−/− mice, CREB3L3−/− mice and combined PPARα/CREB3L3−/− mice after 4 days of ketogenic diet. b Number of genes meeting significance criteria (fold change<− 1.2 or > 1.2 and IBMT P < 0.001) for the comparison between wild-type mice and PPARα−/− mice, CREB3L3−/− mice and combined PPARα/CREB3L3−/− mice after 4 days of ketogenic diet. Principle component analysis (c) and hierarchical clustering (d) of transcriptomics data from liver of wild-type, PPARα−/−, CREB3L3−/−, and combined PPARα/CREB3L3−/− mice after a 4 day ketogenic diet. e Hierarchical biclustering of samples and genes visualized in a heatmap. An IQR (Inter Quartile Range) filter of 0.5 was applied. Red indicates upregulated, blue indicates downregulated