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Fig. 1 | BMC Genomics

Fig. 1

From: Reliable multiplex sequencing with rare index mis-assignment on DNB-based NGS platform

Fig. 1

Mechanisms of index hopping on different sequencing platforms. a Sequencing using DNA nanoball technology is accomplished through Phi29 and RCR linear amplification; each copy is amplified independently using the same template ssCir. In this case, error reads from index hopping cannot accumulate, and most of the signal originates from correct indexes. b Bridge PCR or ExAmp chemistry utilizes exponential amplification, and index hopping can accumulate as amplification proceeds through each cycle, resulting in mis-assigned samples. Green, correct index; red, wrong index

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