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Table 1 Bacterial strains and plasmids used in this study

From: Differential expression profiling of ΔlitR and ΔrpoQ mutants reveals insight into QS regulation of motility, adhesion and biofilm formation in Aliivibrio salmonicida

Bacterial strains or plasmids Description Source
A. salmonicida
 LFI1238 Wild-type, isolated from Atlantic cod [36]
ΔlitR LFI1238 containing an in-frame deletion in litR [27]
ΔrpoQ LFI1238 containing an in-frame deletion in rpoQ [33]
ΔrpoQ-sypQ ΔrpoQ stain with an insertional disruption in sypQ, Cmr This study
ΔrpoQ-sypP ΔrpoQ stain with an insertional disruption in sypP, Cmr This study
ΔrpoQ-sypC ΔrpoQ stain with an insertional disruption in sypC, Cmr This study
 LFI1238-sypQ LFI1238 containing an insertional disruption in sypQ, Cmr This study
 LFI1238-sypP LFI1238 containing an insertional disruption in sypP, Cmr This study
 LFI1238-sypC LFI1238 containing an insertional disruption in sypC, Cmr This study
 LFI1238- pVSV102 A. salmonicida LFI1238 carrying pVSV102, Knr [33]
ΔrpoQ-pVSV102 ΔrpoQ carrying pVSV102, Knr [33]
ΔrpoQ-sypQ-pVSV102 ΔrpoQ-sypQ carrying pVSV102, Knr This study
ΔrpoQ-sypP-pVSV102 ΔrpoQ-sypP carrying pVSV102, Knr This study
ΔrpoQ-sypC-pVSV102 ΔrpoQ-sypC carrying pVSV102, Knr This study
 LFI1238-sypQ-pVSV102 LFI1238-sypQ carrying pVSV102, Knr This study
 LFI1238-sypP-pVSV102 LFI1238-sypP carrying pVSV102, Knr This study
 LFI1238-sypC-pVSV102 LFI1238-sypC carrying pVSV102, Knr This study
E. coli
 C118λpir Helper strain containing pEVS104 [37]
 DH5αλpir E. coli strain containing GFP plasmid pVSV102 [37]
Plasmids
 pNQ705-sypQ pNQ705 containing an internal fragment of sypQ [19]
 pNQ705-sypP pNQ705 containing an internal fragment of sypP [19]
 pNQ705-sypC pNQ705 containing an internal fragment of sypC [19]
 pVSV102 pES213, constitutive GFP, Knr [37]
 pEVS104 R6Korigin, RP4, oriT, trb tra and Knr [64]