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Table 1 Bacterial strains and plasmids used in this study

From: Differential expression profiling of ΔlitR and ΔrpoQ mutants reveals insight into QS regulation of motility, adhesion and biofilm formation in Aliivibrio salmonicida

Bacterial strains or plasmids

Description

Source

A. salmonicida

 LFI1238

Wild-type, isolated from Atlantic cod

[36]

ΔlitR

LFI1238 containing an in-frame deletion in litR

[27]

ΔrpoQ

LFI1238 containing an in-frame deletion in rpoQ

[33]

ΔrpoQ-sypQ

ΔrpoQ stain with an insertional disruption in sypQ, Cmr

This study

ΔrpoQ-sypP

ΔrpoQ stain with an insertional disruption in sypP, Cmr

This study

ΔrpoQ-sypC

ΔrpoQ stain with an insertional disruption in sypC, Cmr

This study

 LFI1238-sypQ

LFI1238 containing an insertional disruption in sypQ, Cmr

This study

 LFI1238-sypP

LFI1238 containing an insertional disruption in sypP, Cmr

This study

 LFI1238-sypC

LFI1238 containing an insertional disruption in sypC, Cmr

This study

 LFI1238- pVSV102

A. salmonicida LFI1238 carrying pVSV102, Knr

[33]

ΔrpoQ-pVSV102

ΔrpoQ carrying pVSV102, Knr

[33]

ΔrpoQ-sypQ-pVSV102

ΔrpoQ-sypQ carrying pVSV102, Knr

This study

ΔrpoQ-sypP-pVSV102

ΔrpoQ-sypP carrying pVSV102, Knr

This study

ΔrpoQ-sypC-pVSV102

ΔrpoQ-sypC carrying pVSV102, Knr

This study

 LFI1238-sypQ-pVSV102

LFI1238-sypQ carrying pVSV102, Knr

This study

 LFI1238-sypP-pVSV102

LFI1238-sypP carrying pVSV102, Knr

This study

 LFI1238-sypC-pVSV102

LFI1238-sypC carrying pVSV102, Knr

This study

E. coli

 C118λpir

Helper strain containing pEVS104

[37]

 DH5αλpir

E. coli strain containing GFP plasmid pVSV102

[37]

Plasmids

 pNQ705-sypQ

pNQ705 containing an internal fragment of sypQ

[19]

 pNQ705-sypP

pNQ705 containing an internal fragment of sypP

[19]

 pNQ705-sypC

pNQ705 containing an internal fragment of sypC

[19]

 pVSV102

pES213, constitutive GFP, Knr

[37]

 pEVS104

R6Korigin, RP4, oriT, trb tra and Knr

[64]