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Fig. 1 | BMC Genomics

Fig. 1

From: Universal human papillomavirus typing by whole genome sequencing following target enrichment: evaluation of assay reproducibility and limit of detection

Fig. 1

Experimental design for evaluation of reproducibility and limit of detection (LOD) of eWGS. To evaluate reproducibility of results, individual indexed libraries were prepared from 16 samples of defined HPV composition on two occasions 10 days apart (Experiments 1 and 2 in Fig. 1) resulting in 2 pooled libraries. Each library was enriched through hybridization with HPV RNA bait and each enriched library was sequenced on two flow cells. Thus 4 replicate results were obtained for each sample, encompassing experimental replicates (reproducibility of producing enriched library) and sequencing replicates (1a, 1b and 2a, 2b). As each defined sample was a pool of 4 to 5 HPV types with copy number ranging from 625 to 1 (composition shown in Table 1), the limit of detection could be assessed from the replicate results

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