Skip to main content

Table 4 Limit of detection of eWGS assay for different HPV types

From: Universal human papillomavirus typing by whole genome sequencing following target enrichment: evaluation of assay reproducibility and limit of detection

HPV

LOD (No. of mapped reads)

LOD (copy number)

No. of mapped reads at 25 copies of HPV genome (mean ± SD)

Concordnacea

HPV11

140.4

25

254 ± 67.7

4/4

HPV16

159.8

25

264.5 ± 84.3

4/4

HPV31

142.4

25

295.3 ± 91.8

4/4

HPV45

163.3

25

496.5 ± 56.7

4/4

HPV52

133.3

25

351.5 ± 116.6

4/4

HPV6

161.1

25

205 ± 17.4

4/4

HPV18

151.4

25

258.3 ± 37.5

4/4

HPV33

161.3

25

329.3 ± 110.2

4/4

HPV58

140.4

25

443.5 ± 56.9

4/4

  1. aConcordance indicates the reproducibility of LOD at 25 copies/HPV genome among the 4 replicates