Fig. 1
From: Development of drug-inducible CRISPR-Cas9 systems for large-scale functional screening
Design and evaluation of drug-inducible sgRNA expression vectors. (a) Schematic for drug-inducible sgRNA expression vectors. Cas9 is constitutively expressed in the cells. EGFP reporter gene is used for the quantification of genome editing activity. PAC encodes puromycin N-acetyltransferase. (b) Representative flow cytometry histograms showing dose-dependent inducible EGFP knockout in MC-38 for tet- (left) and lac- (right) systems. (c) Evaluation of background activity and drug inducible gene knockout efficiency of the inducible sgRNA expression vectors in multiple cell lines. Data represent mean ± SD (n = 3). P values were derived from t tests: *P < 0.05; **P < 0.01; ***P < 0.001; NS, nonsignificant. (d) Calculation of leakiness score and activity score. (e) Heat map of leakiness scores. (f) Heat map of activity scores