Fig. 1

Scr1 regulates a core subset of genes in S. pombe. a Volcano plot of log2 fold change (x-axis) vs -log10 adjusted p-value (y-axis, log scale) for S. pombe protein-coding genes in the scr1⁻ mutant background vs. wild type for the glucose condition. Down-regulated (blue), and up-regulated (red) points indicate genes that met both log2 fold change (− 1 > Log₂FC > 1) and adjusted p-value (P_adj < 0.05) thresholds. Genes of interest are labelled. Known Scr1 regulated genes are shown in bold. b Overlap of genes differentially expressed in the scr1⁻ mutant vs wild type S. pombe on glucose vs sucrose. c GO enrichment analysis of 87 genes differentially expressed in the scr1⁻ mutant in glucose and sucrose. BP = Biological process, CC = cellular component, MF = molecular function. d Overlap of protein-coding genes annotated to Scr1 ChIP-seq peaks in glucose and sucrose conditions. e Enrichment profile of Scr1 surrounding S. pombe gene transcription start sites (TSS) in glucose and sucrose conditions. Solid line indicates the mean while the coloured region defines a 95% confidence interval. f Overlap of differentially expressed genes in scr1⁻ mutant RNA-seq vs a consensus set of protein-coding genes enriched for Scr1 in glucose and sucrose. g Genome browser visualization of Scr1 and RNA PolII^Ser5 (as heatmap) enrichment at the inv1⁺ locus. h Top hit from MEME-ChIP motif analysis of the peak regions from the 32 genes both bound by Scr1 and differentially expressed in the scr1⁻ mutant in the glucose condition. Venn diagram p-values were calculated using the hypergeometric probability distribution