Fig. 3

Loss of scr1⁺ results in ectopic meiosis under carbon and nitrogen replete conditions. a Histogram of wild type and scr1⁻ mutant expression for meiosis genes upregulated in the glucose condition in the scr1⁻ background. Green/red shading indicates pass/fail of Log₂FC, P_adj RNA-seq thresholds and Scr1 enrichment by ChIP-seq (Scr1 ChIP). Only cgs2⁺ appears both directly regulated by Scr1 and significantly upregulated in the scr1⁻ background. b DIC and DAPI microscopy of homothallic wild type (WT h⁹⁰) and scr1Δ (scr1Δ h⁹⁰) S. pombe cells grown on YES at 26 °C. Arrows and inset panel indicate meiotic asci. Scale bars = 20 μm. c Quantification of the proportion of meiotic asci in the scr1Δ h⁹⁰ and WT h⁹⁰ populations out of a total of at least 1000 cells. P-value reflects a two-tailed students T-test assuming unequal variance from three biological replicates. d Representative dissection of scr1Δ h⁹⁰ meiotic tetrads under normal (SPAS 26 °C) or ectopic (YES 26 °C) mating conditions. e Genome browser visualization of Scr1 and RNA PolII^Ser5 enrichment at the cgs2⁺ locus in the glucose, sucrose and glucose-starved “Starved” conditions. f Overview of the S. pombe Git3/Gpa2 glucose sensing pathway