Table 3 Results from metagenomic sequencing of DNA from nasal swabs
From: Rapid cell division of Staphylococcus aureus during colonization of the human nose
Nasal sample | DNA concentration (ng/μl) | Ct (gyrB) | Library concentration (ng/μl) | Total reads | Mapped to S. aureus (%) | Average S. aureus coverage | PTR | Estimated S. aureus doubling time (h) |
|---|---|---|---|---|---|---|---|---|
1 | 0.16 | 26 | 1.95 | 105,786,161 | 1,136,111 (1.1) | 51 | 1.57 | 3.1 |
2 | 0.12 | 27 | 1.12 | 55,786,059 | 2,147,091 (3.8) | 85 | 1.56 | 3.1 |
13 | 0.04 | 29 | 0.59 | 49,621,631 | 1,738,864 (3.5) | 60 | 1.24 | 6.9 |
14 | 0.06 | 28 | 1.32 | 83,741,570 | 1,440,622 (1.7) | 53 | 1.55 | 3.2 |
18 | 0.25 | 28 | 9.11 | 154,201,831 | 681,402 (0.4) | 27 | 1.67 | 2.2 |
32 | 0.37 | 27 | 4.78 | 95,098,143 | 491,336 (0.5) | 23 | 1.54 | 3.3 |
34 | 0.28 | 29 | 8.22 | 124,493,711 | 313,424 (0.3) | 14 | 1.46 | 4.1 |
44 | 0.13 | 23 | 2.78 | 10,457,294 | 958,743 (9.2) | 47 | 1.53 | 3.4 |
45 | 0.29 | 23 | 2.70 | 29,212,511 | 3,832,248 (13.1) | 169 | 1.53 | 3.4 |
46 | 0.78 | 22 | 2.68 | 22,008,815 | 1,111,948 (5.1) | 54 | 1.33 | 5.7 |
| Â | Â | Â | Â | Â | Â | Median PTR: | 1.54 | 3.4 |
6 | 0.09 | 32 | 0.36 | 7,757,779 | 39,355 (0.5) | 1 | n. a. | n. a. |
10 | b. d. | 32 | 0.21 | 1,979,214 | 32,857 (1.7) | 1 | n. a. | n. a. |
19 | 0.06 | 31 | 1.20 | 6,887,014 | 65,255 (0.7) | 2 | n. a. | n. a. |
20 | 0.06 | 33 | 1.21 | 14,195,854 | 75,380 (0.5) | 3 | n. a. | n. a. |