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Fig. 5 | BMC Genomics

Fig. 5

From: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

Fig. 5

Reproducibility analysis of the presented polyA RNA sequencing workflow. (a) Heatmap and (b) PCA analysis representing the variation between sequenced samples. Hierarchical clustering and PCA clustering demonstrate that the technical replicates (Q_2a & Q_2b and A_2a & Q_2b) are the most similar and cluster together. More variance is seen between the sorted samples isolated with different kits (A_2 vs Q_2). All sorted samples cluster away from the unsorted samples. (c) Correlation of all the different samples. Pearson’s correlation coefficient r is calculated on normalized (Deseq2 normalization) log transformed read counts. (d) GO annotated gene sets GO_vasculature_development, GO_chondrocyte_differentiation, GO_mesenchyme_developemnt and GO_neural_crest_cell_differentation are enriched in sorted cells versus unsorted cells

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BMC Genomics

ISSN: 1471-2164

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