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Table 1 Volume of lysis buffer used when its volume was adapted to the amount of sorted cells

From: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

RNA isolation kit # sorted cells Volume of sorted cells (average) Volume of lysis buffer used to collect sorted cells
RNAqueous micro 5000 24.3 μl 350 μl
10,000 48 μl 350 μl
20,000 97 μl 350 μl
30,000 146 μl 350 μl
50,000 240 μl 350 μl
75,000 360 μl 350 μl
100,000 480 μl 700 μl
150,000 730 μl 700 μl
200,000 970 μl 1050 μl
RNeasy plus micro 5000 24.3 μl 100 μl
10,000 48 μl 100 μl
20,000 97 μl 100 μl
30,000 146 μl 200 μl
50,000 240 μl 300 μl
75,000 360 μl 400 μl
100,000 480 μl 500 μl
150,000 730 μl 700 μl
200,000 970 μl 900 μl