Table 1 Volume of lysis buffer used when its volume was adapted to the amount of sorted cells
RNA isolation kit | # sorted cells | Volume of sorted cells (average) | Volume of lysis buffer used to collect sorted cells |
|---|---|---|---|
RNAqueous micro | 5000 | 24.3 μl | 350 μl |
10,000 | 48 μl | 350 μl | |
20,000 | 97 μl | 350 μl | |
30,000 | 146 μl | 350 μl | |
50,000 | 240 μl | 350 μl | |
75,000 | 360 μl | 350 μl | |
100,000 | 480 μl | 700 μl | |
150,000 | 730 μl | 700 μl | |
200,000 | 970 μl | 1050 μl | |
RNeasy plus micro | 5000 | 24.3 μl | 100 μl |
10,000 | 48 μl | 100 μl | |
20,000 | 97 μl | 100 μl | |
30,000 | 146 μl | 200 μl | |
50,000 | 240 μl | 300 μl | |
75,000 | 360 μl | 400 μl | |
100,000 | 480 μl | 500 μl | |
150,000 | 730 μl | 700 μl | |
200,000 | 970 μl | 900 μl |