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Table 3 Star alignment mapping of the sequenced samples

From: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

  A_1 A_2a A_2b A_unsorted Q1 Q_2a Q_2b Q_unsorted
uniquely mapped 88.18% 85.99% 84.29% 83.94% 89.51% 85.49% 85.84% 79.62%
mapped to multiple loci 5.72% 6.70% 6.60% 6.32% 5.49% 6.93% 6.72% 11.12%
mapped to too many loci 0.43% 0.19% 0.22% 0.24% 0.41% 0.19% 0.19% 0.23%
unmapped: too short 5.36% 6.83% 8.57% 9.21% 4.24% 7.14% 7.01% 8.69%
unmapped: other 0.31 0.28 0.31 0.30 0.36 0.24 0.24 0.34
  1. Summary table of the star alignment mapping scores showing percentages of mapped reads per sample. High percentage of uniquely mapped reads is obtained in all samples. Samples starting with an “A” are samples where RNA was isolated with the RNAqueous micro kit. Samples starting with a “Q” are samples where RNA was isolated with the RNeasy plus micro kit. A_1 and Q_1 were sequenced on a different time point then the other samples. No differences were seen between the two RNA isolation kits