Fig. 3

ALFA category plots (raw and normalized) for Ribosome Profiling (Ribo-Seq) data on Mus musculus samples (unpublished data from HLH available on demand). Unt-Rep1 and Unt-Rep2 are two untreated samples while HA-Rep1 and HA-Rep2 are samples treated with harringtonine. Harringtonine is a drug that inhibits the elongation phase of translation, after initiation. Here, ALFA shows that mRNAs are actively translated in the untreated samples (t-test significant at a 1% level with a p-value of 6 × 10^–3) while an expected shift towards the translation start site (i.e. reads spanning the end of the 5’UTR (p-value = 7 × 10^–5) and the start codons (p-value = 1 × 10^–3) thanks to the depth argument set to 4) can be observed in the samples treated with harringtonine.