Fig. 4

ALFA category plots (raw and normalized) for Ribosome Profiling (Ribo-Seq) data on Homo sapiens (unpublished data from AL available on demand) performed with two different procedures for footprinting: treatment with MNase as in [16] or treatment with RNase I as in [17]. As a preprocessing step, rRNA and mtRNA reads were computationally filtered out. The enrichment of signal in intergenic and 3′-UTR regions shows that treatment with MNase seems to produce a substantial increase of the non-protein coding reads compared to RNase I.