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Table 1 Results of the flow cytometric analysis of frozen thawed semen samples

From: A splice donor variant in CCDC189 is associated with asthenospermia in Nordic Red dairy cattle

Animal

Early membrane destabilization

Increased mitochondrial activity

Increased mitochondrial superoxide production

Increased ROS production

Lipid peroxidation degree

Asthenospermic-1

2.0

72.3

24.9

39.0

22.0

Asthenospermic-2

4.2

71.9

25.9

17.8

23.3

Normospermic-1

10.7

47.3

4.9

5.1

3.1

Normospermic-2

8.3

54.3

1.8

6.7

3.2

  1. Proportions (%) of viable spermatozoa post-thawing from two asthenospermic and two normospermic bulls of the Nordic Red breed depicting sperm early membrane destabilization (YO-PRO-1+/PI-), degree of mitochondrial activity (Mitotracker+/PI-), and increased production of mitochondrial superoxide (MitoSOX+/PI-), reactive oxygen species (ROS, CellROX+) and lipid peroxidation (Bodipy-C11 + (green)) assessed using flow cytometry