Skip to main content

Table 1 Results of the flow cytometric analysis of frozen thawed semen samples

From: A splice donor variant in CCDC189 is associated with asthenospermia in Nordic Red dairy cattle

Animal Early membrane destabilization Increased mitochondrial activity Increased mitochondrial superoxide production Increased ROS production Lipid peroxidation degree
Asthenospermic-1 2.0 72.3 24.9 39.0 22.0
Asthenospermic-2 4.2 71.9 25.9 17.8 23.3
Normospermic-1 10.7 47.3 4.9 5.1 3.1
Normospermic-2 8.3 54.3 1.8 6.7 3.2
  1. Proportions (%) of viable spermatozoa post-thawing from two asthenospermic and two normospermic bulls of the Nordic Red breed depicting sperm early membrane destabilization (YO-PRO-1+/PI-), degree of mitochondrial activity (Mitotracker+/PI-), and increased production of mitochondrial superoxide (MitoSOX+/PI-), reactive oxygen species (ROS, CellROX+) and lipid peroxidation (Bodipy-C11 + (green)) assessed using flow cytometry