Fig. 3

Schematic diagram and verification of the new junctions induced by T-DNA integration in the yl genome. a Schematic diagram of the primers’ location around the new junctions. Letters a to o represent the new junctions in the yl genome. b Gel electrophoresis of PCR amplification products. M1, DNA Maker DL15000. M2, DNA Maker DL2000. A1, PCR with primer IS4-R2 and P9182. A2, PCR with primer P9028 and P6345. A3, PCR with primer P6008 and P3895. A4, PCR with primer P6345 and IS5-F2. B1, PCR with primer IS2-F and P3054. B2, PCR with primer P3054 and IS3-R1. C1, PCR with primer IS6-R2 and P6008. C2, PCR with P6345 and IS6-F. c Verification of the junction between Chr2–4 and Chr8–1. Gel electrophoresis showed PCR amplification products with primer TB-R and IS3-F. N. Negative control (water)