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Table 2 Mutations associated with resistance to amoxicillin in strains with a previously acquired resistance to enrofloxacin, kanamycin, or tetracycline

From: Effects of a previously selected antibiotic resistance on mutations acquired during development of a second resistance in Escherichia coli

Gene product Gene Mutation Pop. frequency Strains
Transcriptional regulator acrR L204R, Del C205 0.98–1 E1/4, K2/4 T2/4
B-lactamase ampC C35A (promoter) 1 E4/4, K3/4, T1/4
C-11 T (promoter) 1 E4/4, K3/4, T1/4
Ins T after C4379837 (promoter) 1 K1/4
Ins T after A4379015 (promoter) 1 T1/4
Citrate lyase citF G432A 1 E4/4, K3/4, T1/4
Glutamate synthase subunit gltD A80A 0.18 T1/4
L-glyceraldehyde 3-phosphate reductase gpr L180F 1 T2/4
CPS-53 glucosyl transferase gtrS V351 V 0.96 K1/4
Acetolactate synthase/acetohydroxybutanoate synthase subunit ilvH G52C 0.60–1 E2/4, K2/4, T1/4
Branched chain amino acid/phenylalanine ABC transporter membrane subunit livH G239R 0.18 E1/4
Intermembrane phospholipid transport system subunit mlaF Ins AIT between T38-A39 0.65–0.66 T2/4
Large conductance mechanosensitive channel mscL L45Q 0.98 K1/4
Mismatch repair protein mutL W390 L 0.11–0.2 T2/4
Transcriptional regulator ompR E88A 1 E4/4, K3/4, T1/4
RNA polymerase subunit α rpoA C269S 1 E1/4, K1/4
RNA polymerase subunit β rpoB E564A 0.15 T1/4
Sigma 70 rpoD D445V 1 E4/4
Alternative sigma factor σS rpoS E16Stop 0.96–1 K2/4, T1/4
Del Q59-E330 0.91–1 T2/4
Potassium ion transporter trkG G159D 1 K1/4
  1. Del = deletion, Ins = insertion, underlined letters and numbers indicate nucleotides and their genomic position. Population frequency indicates the mutational frequency in the sequenced population. Strains indicates the prevalence of a mutation within different replicates. E: population with previously acquired resistance to enrofloxacin, K: population with previously acquired resistance to kanamycin, T: population with previously acquired resistance to tetracycline. X/Y: X indicates number of replicates with particular mutation, Y indicates total number of replicates that were sequenced. Mutations associated with resistance in a wild-type background are shown in bold. Mutations associated with the previous resistance have been omitted from the table