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Table 3 Mutations associated with resistance to enrofloxacin in strains with a previously acquired resistance to amoxicillin, kanamycin, or tetracycline

From: Effects of a previously selected antibiotic resistance on mutations acquired during development of a second resistance in Escherichia coli

Gene product

Gene

Mutation

Pop. frequency

Strains

Multidrug efflux pump membrane fusion lipoprotein/transcriptional regulator

acrA/acrR*

5′ UTR (Del 485,695–711)

1

T1/3

Transcriptional regulator

acrR

Del 485,885–11 (FS42)

1

A3/4, K1/4, T2/3

Aconitate hydratase B/2-methylisocitrate dehydratase

acnB

G362 V, Del L363-V865

1

T1/3

Alkyl hydroperoxide reductase

ahpC

Del F38

0.40–1

A2/4, T1/3

Crotonobetainyl-CoA reductase

caiA

C154A, F155P, I156T, T157P, S158R, S159 T, A160S, Y161W, Del T162-R380

1

A2/4, T1/4

ATP-dependent helicase

dinG

Del Q641-R716

0.89

A1/4

Glutamate synthase subunit

gltB

5′ UTR (C3354487T)

1

K1/4, T2/3

DNA gyrase subunit A

gyrA

S83 L

1

A4/4, E1/4, T3/3

D87G

1

A3/4, K2/4, T1/3

D87N

1

K2/4, T2/3

Branched chain amino acid/phenylalanine ABC transporter membrane subunit

livF

G239F

0.87

T1/4

Mismatch repair protein

mutL

W390 L

0.09–0.14

K1/4, T2/3

Phosphorelay protein

npr

L23R

1

T1/3

Phenylacetate degradation protein

paaD

A8S

1

T1/3

Putative 2,3-dehydroadipyl-CoA hydratase

paaF

V35 L

1

T1/3

DNA topoisomerase IV subunit A

parC

S80R

0.94–1

A3/4, K4/4, T3/3

DNA topoisomerase IV subunit B

parE

Ins Q458

0.97–1

A3/4, K2/4, T2/3

E459Q

0.03

A1/4

S425P

0.81

K2/4

Ins S after A457

0.95

T1/3

tRNA-Phe

pheU

5′ UTR (G4362633 T)

1

K1/4

Cryptic proline/histidine transporter

proY

A235S

1

A3/4, K1/4, T2/3

Pyridine nucleotide transhydrogenase subunit β

ptnB

V434 V

0.57

K1/4

Phosphate acetyltransferase

pta

Del T431-Q714

0.56

K1/4

Sensory histidine kinase

rcsC

E44K

1

K2/4

Truncated RNase PH

rph

Del R676

1

A2/4, T1/3

S10 subunit of 30S

rpsJ

5′ UTR (G3453306 T)

1

K1/4, T2/3

Bifunctional (p) ppGpp synthase/hydrolase

spoT

Ins C after T3815879 (FS223)

1

A2/4, T1/4

Transcriptional regulator

soxR

T113S, del 4,277,899–903 (FS14)

1

A3/4, K1/4, T1/3

Ins G136

0.15

K1/4

L139F, del T after C4277882

0.85

K1/4

A146E

1

K1/4

Del R139-N154

1

K1/4

Succinyl-CoA synthetase subunit β

sucC

Del A218-N221

0.51

K1/4

DNA-binding transcriptional activator

ybaO

T33P

1

T1/4

Putative RNA binding protein

yciO

N64Y

1

A1/4

Putative transport protein/Putative inner membrane protein

ybjL/ybjM *

5′ UTR (A889923G)

1

A3/4, K1/4, T2/3

  1. Del = deletion, Ins = insertion, underlined letters and numbers indicate nucleotides and their genomic position, * indicates that mutation could affect either gene. Population frequency indicates the mutational frequency in the sequenced population. Strains indicates the prevalence of a mutation within different replicates. E: population with previously acquired resistance to enrofloxacin, K: population with previously acquired resistance to kanamycin, T: population with previously acquired resistance to tetracycline. X/Y: X indicates number of replicates with particular mutation, Y indicates total number of replicates that were sequenced. Mutations associated with resistance in a wild-type background are shown in bold. Mutations associated with the previous resistance have been omitted from the table
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BMC Genomics

ISSN: 1471-2164

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