Table 2 DEGs involved in GS biosynthesis. FC is the short name of fold change of gene expression. Log2 FC1 denotes the values in HG vs HGT, Log2 FC2 denotes the values in LG vs LGT, and Log2 FC3 denotes the values in HGT vs LGT. “Inf” represented no transcript level of DEG in control sample, and “-Inf” represented no transcript level of DEG in treated sample

Chain elongation

BCAT4

Bo3g073430

Methionine aminotransferase BCAT4

–

–

4.17

Bo5g113720

Methionine aminotransferase BCAT4

–

–

5.22

MAM1

Bo2g161100

Methylthioalkylmalate synthase 1, chloroplastic (Precursor)

–

–

4.61

Bo7g098000

Methylthioalkylmalate synthase 2, chloroplastic (Precursor)

–

–

5.95

Core structure formation

CYP79F1

Bo5g021810

Dihomomethionine N-hydroxylase

–

−2.96

4.98

CYP79B1

Bo1g002970

Cytochrome P450 79B1

–

− 2.81

–

CYP83A1

Bo4g130780

Cytochrome P450 83A1

–

–

4.35

Bo4g191120

Cytochrome P450 83A1

–

–

–

SUR1

Bo7g113100

S-alkyl-thiohydroximate lyase SUR1

–

–

2.85

UGT74B1

Bo5g041080

UDP-glycosyltransferase 74B1

2.78

–

–

UGT74C1

Bo4g049480

UDP-glycosyltransferase 74C1

–

–

4.83

Bo4g177530

UDP-glycosyltransferase 74C1

–

–

–

SOT

Bo6g067810

Cytosolic sulfotransferase 1

–

–

Inf

Bo6g097270

Cytosolic sulfotransferase 1

–

–

-Inf

Bo6g118380

Cytosolic sulfotransferase 16

–

–

−2.51

Bo2g080910

Cytosolic sulfotransferase 16

–

–

− 2.51

FMO

Bo8g108370

Flavin-containing monooxygenase FMO GS-OX-like 2

–

–

− 2.11

Bo9g028520

Flavin-containing monooxygenase FMO GS-OX-like 3

−3.98

−2.33

–

Bo7g098490

Flavin-containing monooxygenase FMO GS-OX-like 8

–

–

2.18

Bo9g174970

Flavin-containing monooxygenase FMO GS-OX-like 9

−2.03

–

–

CYP81F1

Bo2g032590

Cytochrome P450 81F1

−2.32

−4.39

–

Bo1g004730

Cytochrome P450 81F1

–

−2.48

–

Bo9g131960

Cytochrome P450 81F1

–

−2.64

−2.72

Side chain

Modification

degradation

AOP2

Bo9g006220

glucosinolate GS-Alk

–

–

3.18

TGG

Bo8g039420

myrosinase, thioglucoside glucohydrolase

–

–

3.96