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Fig. 1 | BMC Genomics

Fig. 1

From: A cmap-enabled gene expression signature-matching approach identifies small-molecule inducers of accelerated cell senescence

Fig. 1

Radiation and p21 overexpression induce similar senescence phenotypes. a SA-β-gal assay of MCF7GFP-IBD cells, 5 days after 6 Gy irradiation, compared to MCF7p21 cells, 5 days after induction with doxycycline, displays activation of beta-galactosidase activity (blue staining) under both conditions. Scale bar = 20 μm. b Immunofluorescence staining for phospho-H2AX in MCF7GFP-IBD cells demonstrates nuclear foci 3 h after 6 Gy irradiation while MCF7p21 cells display no foci 3 h after induction with doxycycline. Scale bar = 20 μm. c Immunofluorescence staining for p21 in MCF7GFP-IBD cells, 5 days after 6 Gy irradiation, compared to MCF7p21 cells, 5 days after induction with doxycycline, demonstrates increased expression under each condition. Untreated controls are shown for each assay. Scale bar = 20 μm.

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