Senescence phenotypes induced by compounds identified by cmap analysis. a SA-β-gal assay in MCF7p21 cells treated with the cmap screen hits, assessed 5 days after adding the indicated compounds. Scale bar = 20 μm. b GFP-IBD foci in MCF7GFP-IBD treated with radiation or cmap screen hits. Nuclear GFP foci indicating double strand breaks were observed at 3 h and 24 h after 6 Gy irradiation. Treatment with daunorubicin, etoposide, withaferin A, or phenoxybenzamine produced nuclear GFP foci at 24 h. Treatment with fulvestrant or vorinostat failed to induce foci above background. Scale bar = 20 μm.