Fig. 2
From: An improved protein extraction method applied to cotton leaves is compatible with 2-DE and LC-MS
Chromatograms of the signals from HPLC fractionation (a) and the total ionic chromatograms from MS/MS (b). The digestion products of the TCA, BPP and TCA-B methods were used for HPLC fractionation (left) using a C18 reversed-phase column with a 65-min gradient. The UV absorbance from 5 to 55 min was recorded at a wavelength of 214 nm. The total ionic chromatograms (right) were acquired using an AB Sciex TripleTOF 5600 plus system, and the samples were injected and eluted using a 95-min gradient