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Fig. 5 | BMC Genomics

Fig. 5

From: Transcriptional profiling and pathway analysis reveal differences in pituitary gland function, morphology, and vascularization in chickens genetically selected for high or low body weight

Fig. 5

RT-qPCR confirmation of expression profiles determined by microarray and SOMs analysis for select DEGs. Microarray (a-f, top graph) and RT-qPCR (a-f, bottom graph) data are shown for each gene on post-hatch weeks 1, 3, 5, and 7. For RT-qPCR data, levels of mRNA for each gene were normalized to levels of GAPDH mRNA. Values (mean + SEM; n = 4 birds per line) are expressed relative to the age and line with the highest expression level for each technique (set to 100% prior to log2-transformation for statistical analysis). The RT-qPCR data for TSHβ mRNA (d, bottom) are also shown in Fig. 2b. When the two-way ANOVA demonstrated a significant line-by-age interaction (P ≤ 0.05), the presence of an asterisk (*) denotes a significant difference between the lines at the indicated age (P ≤ 0.05). Significant main effects of line and/or age (P ≤ 0.05) are indicated at the top of each graph when the line-by-age interaction was not significant (P > 0.05)

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