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Table 5 Theoretical limit of detection for kinase gene fusion events

From: Comprehensive kinome NGS targeted expression profiling by KING-REX

Gene expression value of FL kinase in sample 2 (GE2)

Gene expression value of kinase gene fusion in sample 1 (GE1)

GE1 = 15

GE1 = 14

GE1 = 13

GE1 = 12

GE1 = 11

GE1 = 10

GE1 = 9

GE1 = 8

GE1 = 7

GE1 + 0

81.25

81.25

81.25

81.25

87.50

87.50

87.50

87.50

93.75

GE1–1

62.50

62.50

62.50

62.50

68.75

75.00

75.00

75.00

93.75

GE1–2

50.00

50.00

43.75

43.75

50.00

56.25

62.50

68.75

87.50

GE1–3

31.25

31.25

31.25b

37.50

37.50

37.50

43.75

62.50

81.25

GE1–4

18.75

18.75

18.75

25.00

31.25

31.25

37.50

50.00

75.00

GE1–5

12.50

12.50

12.50

18.75

18.75

25.00

31.25

43.75

68.75

GE1–6

6.25

6.25

12.50

12.50

12.50

18.75

31.25

43.75

68.75

GE1–7

6.25

6.25

6.25

12.50

12.50

18.75

25.00

37.50

62.50

GE1–8

6.25

6.25

6.25

6.25

12.50

12.50

25.00

37.50

 

GE1–9

6.25

6.25

6.25

6.25

12.50

12.50

25.00

  

GE1–10

6.25

6.25

6.25

6.25

6.25

12.50

   

GE1–11

6.25

6.25

6.25

6.25

6.25a

    

GE1-12

6.25

6.25

6.25

6.25

     

GE1–13

6.25

6.25

6.25

      

GE1–14

6.25

6.25

       

GE1–15

6.25

        
  1. ‘Theoretical’ limit of detection (expressed as the percentage of sample containing the gene fusion vs. the background full length (FL) sample) for the detection of kinases involved in gene fusions with varying expression levels of the fused kinase domain and of the full length WT kinase (background), calculated using ASSAY IN and ASSAY OUT values as described in M&M. GE1 Gene expression level of the fused kinase in sample1, GE2 Gene expression level of the full length kinase (background) in sample 2
  2. atheoretical limit of detection calculated in the same case of ROS1 kinase in U-118 MG (GE1 = 11) diluted in KARPAS 299 not expressing ROS1 (GE2 = 0)
  3. btheoretical limit of detection calculated in the same case of ALK kinase in KARPAS 299 (GE1 = 13) diluted in U-118 MG expressing full length ALK (GE2 = 10)