Comprehensive kinome NGS targeted expression profiling by KING-REX

BMC Genomics

Table 6 KING-REX performance with heat degraded RNA

Time (minutes)	RNA quality		Library preparation and sequencing			Gene expression profiling and fusion detection
Time (minutes)	RIN	DV200% (if RIN < 8)	Replicates	Library Conc (ng/μl)	PF Reads (10⁶)	R² with respect T = 0	PVAL
0	7.6	94	A	4.2	2.14	1.00	1.21E-143
0	7.6	94	B	6.6	2.00	1.00	1.21E-143
10	3	90	A	5.5	1.87	0.99	2.14E-223
10	3	90	B	6.9	1.87	0.99	2.14E-223
20	2.2	69	A	2.8	1.80	0.97	5.29E-082
20	2.2	69	B	0.9	0.70	0.97	5.29E-082
60	2.6	10	A	0.4	0.04	0.78	NA
60	2.6	10	B	0.4	0.03	0.78	NA

RNA parameters, library concentration and number of reads obtained from the sequencing of heat degraded KM-12 RNA samples at different time points. Correlation of kinase gene expression profiles at different time point vs. time = 0 (T = 0) and p-value relative to the detection of the TPM3-NTRK1 kinase gene fusion are reported (PF = Reads passing filter). The experiment was performed in duplicate

ISSN: 1471-2164