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Fig. 6 | BMC Genomics

Fig. 6

From: Genomic analyses of aminoacyl tRNA synthetases from human-infecting helminths

Fig. 6

Enzymatic assays of Onchocerca volvulus PRS. a) Purified OvPRS on SDS-PAGE b) Aminoacylation assay using malachite green dye (53). Linearity of the assay with respect to enzyme concentration. c) Assay linearity with respect to time, red shows control without enzyme and the blue is reaction in presence of enzyme. d, e) Proline Km determination in the presence of a saturating concentration of ATP (200 μM), ATP Km determination in the presence of saturating concentration of proline (1 mM). f) Enzyme inhibition with halofuginone using 50 μM proline, 150 μM ATP and 150 nM OvPRS enzyme. The IC50 of halofuginone for inhibition of OvPRS is estimated to be ~ 30 nM

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