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Fig. 2 | BMC Genomics

Fig. 2

From: Tissue specific human fibroblast differential expression based on RNAsequencing analysis

Fig. 2

Lineage characterization of fibroblast cultures. Brightlight microscopy of vocal fold (a), trachea (b), and lung (c) fibroblast cultures exhibiting homogenous populations with classic elongated, spindle-shape morphology. VFF negative for markers of cytokeratin 19 (d), mounted with propidium iodine, von Willebrand (vWF) (e), and α-actinin (f), mounted with DAPI. Epithelial cells exhibit positive (g) expression for cytokeratin 19. Endothelial cells exhibit positive (h) expression for vWF. Skeletal cells exhibit positive (i) expression for α-actinin. Experiments were performed in triplicates and with negative controls, both in the absence of primary antibodies and with known negative cell types. Brightfield photos taken at 10x and immunofluorescence photos taken at 20x magnification. Scale bar represents 100 μm

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