Fig. 2

Transcriptional regulation of different domains of BmOVO-1 at the N-terminus on Bmotu promoter. a Schematic of truncated BmOVO-1. Upper, the domains of BmOVO-1, A represents acidic domain, B represents alkaline domain, Z represents zinc-finger domain, Number on domain means number of amino acid residues in a domain; Lower, the truncated BmOVO-1. BmOVO-T2, A1 region of BmOVO-1 was deleted; BmOVO-T3, A1-A2 region of BmOVO-1 was deleted; BmOVO-TB1, A1-A3 region of BmOVO-1 was deleted; BmOVO-T4, A1-B1 region of BmOVO-1 was deleted; BmOVO-T5, A1-A4 region of BmOVO-1 was deleted; BmOVO-TB2, A1-A5 region of BmOVO-1 was deleted; BmOVO-T1, BmOVO-2 was fused with A1 domain. b, c Plasmids with different truncated Bmovo-1(1 × 10¹¹ copies) and BmOVO-T1(1 × 10¹¹ copies) were respectively cotransfected into BmN cells (10⁵) with plasmid pRL-TK (1 × 10¹⁰ copies). The co-transfected BmN cells with pFast-potu5-Luc-ie1-Bmovo1(1 × 10¹¹ copies) and pRL-TK (1 × 10¹⁰ copies) plasmids was used as a control. Luciferase activities in the cells were determined at 60 h post-transfection, 100 μg protein from the lysed cells was used for luciferase assay. *p < 0.05, **p < 0.01, ***p < 0.001